Journal: Mediators of Inflammation

Article Title: The Role of EGFR/PI3K/Akt/cyclinD1 Signaling Pathway in Acquired Middle Ear Cholesteatoma

doi: 10.1155/2013/651207

Figure Lengend Snippet: The cultured cells grew into monolayer and showed a polygonal “cobblestone-like” appearance (a). The cultured cells showed a positive reaction to cytokeratins by immunofluorescence, and the signal was detected in cytoplasm (b).

Article Snippet: The cells were then incubated with primary mouse antibody against cytokeratins (1 : 150 dilution; Boster, China) at 37°C for 90 min. After incubation, the cells were washed in PBS and incubated with TRITC-labeled goat-anti-mouse secondary antibody (1 : 50 dilution; Boster, China) at 37°C for 60 min. Coverslips were finally mounted for observation.

Techniques: Cell Culture, Immunofluorescence

Journal: Diagnostic Pathology

Article Title: Concurrent hypermethylation of DNMT1, MGMT and EGFR genes in progression of gliomas

doi: 10.1186/1746-1596-7-8

Figure Lengend Snippet: Representative methylation analysis of CpG islands in the MGMT, EGFR and DNMT1 promoter in GL samples of cases 6, 12 and 16 . C, undigested DNA samples; P, β2- microglobulin as a positive control; N, no DNA sample as a negative control; U, unmethylated DNA samples; M, methylated DNA samples, m, molecular weight marker

Article Snippet: After microwave antigen retrieval procedure the sections were incubated with the anti-EGFR monoclonal antibody (DAKO Glostrup, Denmark, RTU), anti- DNMT1 monoclonal antibody (BIOZOL, Germany 1:500) and anti-MGMT monoclonal antibody (Novobius, USA 1:50).

Techniques: Methylation, Positive Control, Negative Control, Molecular Weight, Marker